Device

Part:BBa_K1420006:Design

Designed by: Stephen C. Heinsch   Group: iGEM14_Minnesota   (2014-10-09)


phsABC Generator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 135
    Illegal BglII site found at 2197
    Illegal BamHI site found at 141
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 249
    Illegal AgeI site found at 2772
    Illegal AgeI site found at 3555
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Cloned into pBBRBB-eGFP (https://www.addgene.org/32549/) via BamHI and XhoI restriction sites. Amplified region containing modified lac promoter and phsABC coding region flanked by BioBrick prefix and suffix sequences.


Source

N/A

References